Year of publication:
2009
Journal name:
Blood
Role for ADAP in shear flow-induced platelet mechanotransduction.
http://www.ncbi.nlm.nih.gov –
Binding of platelets to fibrinogen via integrin alphaIIbbeta3 stimulates cytoskeletal reorganization and spreading. These responses depend on tyrosine phosphorylation of multiple proteins by Src family members and Syk. Among Src substrates in platelets is ADAP, an adapter with three potential binding partners, SLP-76, VASP and SKAP-HOM. During studies of platelet function under shear flow, we discovered that ADAP(-/-) mouse platelets, unlike ADAP(+/+) platelets, formed unstable rather than stable thrombi in response to carotid artery injury. Read more »
Inhibition of Cell Migration, Spreading, and Focal Adhesions by Tumor Suppressor PTEN
http://www.sciencemag.org –
The tumor suppressor PTEN is a phosphatase with sequence similarity to the cytoskeletal protein tensin. Here the cellular roles of PTEN were investigated. Overexpression of PTEN inhibited cell migration, whereas antisense PTEN enhanced migration. Integrin-mediated cell spreading and the formation of focal adhesions were down-regulated by wild-type PTEN but not by PTEN with an inactive phosphatase domain. PTEN interacted with the focal adhesion kinase FAK and reduced its tyrosine phosphorylation. Overexpression of FAK partially antagonized the effects of PTEN. Read more »
Year of publication:
1998
Journal name:
Science