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Extracellular matrix, mechanotransduction and structural hierarchies in heart tissue engineering
http://www.childrenshospital.org –
The spatial and temporal scales of cardiac organogenesis and pathogenesis make engineering of
artificial heart tissue a daunting challenge. The temporal scales range from nanosecond conformational changes responsible for ion channel opening to fibrillation which occurs over Read more »
Year of publication:
2007
Journal name:
Philosophical Transaction of Royal Society B
Mechanotransduction in Single Cardiac Myocyte Studied Using Laser Tweezers and FRET
http://www.sciencedirect.com –
Deletion or mutation of a variety of proteins localized at cell-matrix and cell-cell junctions, such as vinculin and its splice-variant metavinculin, can lead to dilated cardiomyopathy in mice and humans, leading some to hypothesize that these molecules are involved in mechanotransmission or mechanotransduction in the heart.
To investigate cardiac mechanotransduction mechanisms in single cells, we have combined laser tweezers with a fluorescence resonance energy transfer (FRET) biosensor to apply localized forces and probe localized signaling events in isolated mouse ventricular myocytes. Read more »
Year of publication:
2009
Journal name:
Biophysical Society
Pressure-Induced Vascular Oxidative Stress Is Mediated Through Activation of Integrin-Linked Kinase 1/βPIX/Rac-1 Pathway
http://hyper.ahajournals.org –
High blood pressure induces a mechanical stress on vascular walls and evokes oxidative stress and vascular dysfunction. The aim of this study was to characterize the intracellular signaling causing vascular oxidative stress in response to pressure. In carotid arteries subjected to high pressure levels, we observed not only an impaired vasorelaxation, increased superoxide production, and NADPH oxidase activity, but also a concomitant activation of Rac-1, a small G protein. Read more »
Year of publication:
2009
Journal name:
Hypertension
A mechanosensitive transcriptional mechanism that controls angiogenesis
http://www.ncbi.nlm.nih.gov:80 –
Angiogenesis is controlled by physical interactions between cells and extracellular matrix as well as soluble angiogenic factors, such as VEGF. However, the mechanism by which mechanical signals integrate with other microenvironmental cues to regulate neovascularization remains unknown. Here we show that the Rho inhibitor, p190RhoGAP, controls capillary network formation in vitro and retinal angiogenesis in vivo by modulating the balance of activities between two antagonistic transcription factors – TFII-I and GATA2 – that govern gene expression of the VEGF receptor, VEGFR2. Read more »
Year of publication:
2009
Journal name:
Nature
Cardiac mechanotransduction: from sensing to disease and treatment
http://www.sciencedirect.com –
In heart muscle a mechanical stimulus is sensed and transformed into adaptive changes in cardiac function by a process called mechanotransduction. Adaptation of heart muscle to mechanical load consists of neurohumoral activation and growth, both of which decrease the initial load. Under prolonged overload this process becomes maladaptive, leading to the development of left ventricular hypertrophy and ultimately to heart failure. Read more »
Year of publication:
2001
Journal name:
Trends in Pharmacological Sciences
Mechanotransduction in Cardiac Myocytes
http://www3.interscience.wiley.com –
Cardiac myocytes react to diverse mechanical demands with a multitude of transient and long-term responses to normalize the cellular mechanical environment. Several stretch-activated signaling pathways have been identified, most prominently guanine nucleotide binding proteins (G-proteins), mitogen-activated protein kinases (MAPK), Janus-associated kinase/signal transducers and activators of transcription (JAK/STAT), protein kinase C (PKC), calcineurin, intracellular calcium regulation, and several autocrine and paracrine factors. Multiple levels of crosstalk exist between pathways. Read more »
Year of publication:
2006
Journal name:
Annals of the New York Academy of Sciences

