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Mechanotransduction in Single Cardiac Myocyte Studied Using Laser Tweezers and FRET

http://www.sciencedirect.com
Year of publication: 
2009
Journal name: 
Biophysical Society
Deletion or mutation of a variety of proteins localized at cell-matrix and cell-cell junctions, such as vinculin and its splice-variant metavinculin, can lead to dilated cardiomyopathy in mice and humans, leading some to hypothesize that these molecules are involved in mechanotransmission or mechanotransduction in the heart. To investigate cardiac mechanotransduction mechanisms in single cells, we have combined laser tweezers with a fluorescence resonance energy transfer (FRET) biosensor to apply localized forces and probe localized signaling events in isolated mouse ventricular myocytes. Read more »
esoohoo's picture
Created by esoohoo 2 years 6 weeks ago
Category: Cardiovascular   Tags:
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Signal transduction pathways involved in mechanotransduction in bone cells

http://linkinghub.elsevier.com
Year of publication: 
2006
Journal name: 
Biochemical and Biophysical Research Communications 349 (2006) 1–5
Several in vivo and in vitro studies with different loading regimens showed that mechanical stimuli have an influence on proliferation and differentiation of bone cells. Prerequisite for this influence is the transduction of mechanical signals into the cell, a phenomenon that is termed mechanotransduction, which is essential for the maintenance of skeletal homeostasis in adults. Read more »
1

Collagen-Based Mechanical Anisotropy of the Tectorial Membrane: Implications for Inter-Row Coupling of Outer Hair Cell Bundles

http://www.plosone.org
Year of publication: 
2009
Journal name: 
PLoS ONE
Background: The tectorial membrane (TM) in the mammalian cochlea displays anisotropy, where mechanical or structural properties differ along varying directions. The anisotropy arises from the presence of collagen fibrils organized in fibers of ~1 µm diameter that run radially across the TM. Mechanical coupling between the TM and the sensory epithelia is required for normal hearing. However, the lack of a suitable technique to measure mechanical anisotropy at the microscale level has hindered understanding of the TM's precise role. Methodology/Principal Findings: Read more »
dorothy_tulanont's picture
Created by dorothy_tulanont 2 years 6 weeks ago
Category: Mechanosensors in Sensory Cells   Tags:
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1

Nonequilibrium Mechanics of Active Cytoskeletal Networks

http://www.sciencemag.org
Year of publication: 
2007
Journal name: 
Science
Cells both actively generate and sensitively react to forces through their mechanical framework, the cytoskeleton, which is a nonequilibrium composite material including polymers and motor proteins. We measured the dynamics and mechanical properties of a simple three-component model system consisting of myosin II, actin filaments, and cross-linkers. Read more »
csi's picture
Created by csi 2 years 6 weeks ago
Category: Cytoskeletal Dynamics   Tags:
1

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Crossing the Nuclear Envelope: Hierarchical Regulation of Nucleocytoplasmic Transport

http://www.sciencemag.org
Year of publication: 
2007
Journal name: 
Science
Transport of macromolecules between the nucleus and cytoplasm is a critical cellular process for eukaryotes, and the machinery that mediates nucleocytoplasmic exchange is subject to multiple levels of control. Regulation is achieved by modulating the expression or function of single cargoes, transport receptors, or the transport channel. Each of these mechanisms has increasingly broad impacts on transport patterns and capacity, and this hierarchy of control directly affects gene expression, signal transduction, development, and disease. Read more »

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